We want to hear from you. The .gov means its official. INFLUENCE OF pH AND OF CERTAIN OTHER CONDITIONS ON THE STABILITY OF THE INFECTIVITY AND RED CELL AGGLUTINATING ACTIVITY OF INFLUENZA VIRUS. CENTRIFUGATION AND ULTRAFILTRATION STUDIES ON ALLANTOIC FLUID PREPARATIONS OF INFLUENZA VIRUS. Attenuated negative strand viruses with altered interferon antagonist activity for use as vaccines and pharmaceuticalsAttenuated negative strand viruses with altered interferon antagonist activity for use as vaccines and pharmaceuticals .. .. . Addresses across the entire subnet were used to download content in bulk, in violation of the terms of the PMC Copyright Notice. The virus was shown not to lose infectivity very rapidly in sucrose solutions. Support Center Find answers to questions about products, access, use, setup, and administration. Seasonal influenza infection is associated with 200,000-500,000 deaths each year, particularly in young children, immunocompromised patients and the elderly. Federal government websites often end in .gov or .mil. Bethesda, MD 20894, Web Policies The .gov means its official. 2 a). Careers. Morphologial and structural analysis of influenza virus PR8 strain at pH 5.5 In this condition, most virions have no apparent structural changes ( Fig. Sedimentation studies in sucrose solutions of varying densities showed a non-linear dependence of sedimentation rate upon solvent density, indicating that the density in solution increases with increasing sugar concentration. Cellular infiltrate and cytokines in BAL or lung homogenate Gross pathology scores Validation Data Figure 1. Electron micrographs showed slightly irregular, circular particles with an average diameter of 115 mmicro and a standard deviation of the distribution of diameters of 15 per cent. The invention relates to a nucleic acid construct encoding at least three RNA molecules comprising a double-stranded region, wherein the RNA molecules reduce influenza A virus replication in an animal cell and/or reduce production of infectious influenza A virus particles in an animal cell and/or reduce the expression of an influenza A virus polypeptide in an influenza A virus infected animal . Introduction Although aluminum in virtually all currently adjuvant human vaccines is used to help increase the humoral or cellular immune responses to an antigen, it usually induces a Th2 antibody dominated response. 1944 Mar 1;79(3):301-17. doi: 10.1084/jem.79.3.301. PMC The intrinsic viscosities, calculated on the basis of concentrations expressed as grams per cubic centimeter, of several highly purified preparations were determined to be between 11.3 and 16.5. For example, infection of mice with the pathogenic PR8 strain of influenza virus (A/Puerto Rico/8/1934 [H1N1]) at a sublethal dose results in spread of the virus to the heart and replication to low levels ( 9, 10 ), but the resulting myocarditis is mild and resolves quickly as the virus is cleared ( 9 ). The virus was shown not to lose infectivity very rapidly in sucrose solutions. A combination of high-yield mutations from these screens led to a PR8 backbone that improved the titres of H1N1, H3N2, H5N1 and H7N9 vaccine viruses in African green monkey kidney and. Click here to visit your profile and update your email address and/or resend the verification message. Product category Viruses Product type 75N93019C00076, awarded to the University of Chicago. If you have questions or need assistance, please contact us at [email protected]. Search for other works by this author on: Copyright, 1944, by The Rockefeller Institute for Medical Research New York, This site uses cookies. 8600 Rockville Pike However, reassortants with H5-derived HA with a polybasic cleavage site are potentially hazardous for animal health. Iota-carrageenan inhibits influenza virus-induced plaque formation on MDCK cells. Sedimentation studies in sucrose solutions of varying densities showed a non-linear dependence of sedimentation rate upon solvent density, indicating that the density in solution increases with increasing sugar concentration. The site is secure. Together, these data demonstrate that SERINC5 . However, some differences from pH 7.2 can be observed from the tomograms. . (PR8), a variant with very different properties, and chimeric proteins between two, we identified amino acids 68-71 have important roles in protein stability, subcellular . The specific volume was determined with a pycnometer to be 0.79. This virus was named Influenza B and the inactivated. -, J Exp Med. The polymer exhibited virus-inhibiting properties with an IC 50 = 1 g/mL and a selectivity index of 302. FOIA Proposed mechanism of influenza virus inactivation by N, N -dodecyl,methyl-PEI coatings. 1944 Feb 1;79(2):197-203 . CiteSeerX - Document Details (Isaac Councill, Lee Giles, Pradeep Teregowda): During the past decade several studies on the physical properties of various strains of influenza virus have been reported. Prophylactic intranasal administration of the polymer in a murine model of influenza pneumonia completely prevented the virus-induced death of animals, whereas the level of mortality in the placebo group was 90%. The extrapolated value for one preparation was 722 Svedberg units and the value for another preparation was 658 units. Accessibility Accessibility To examine that our model can recognize influenza CPE induced by different influenza virus strains, we collected about 120 image of MDCK cells infected by pandemic H1N1/09, H3N2, and influenza B at 16 . The variation of sedimentation rate with concentration was shown to be due entirely to the variation of solution viscosity with concentration. }, author={J. Bronwyn Bateman and A. Zellner and M. Stuart Davis and P A McCAFFREY}, journal={Archives of . sharing sensitive information, make sure youre on a federal Bookshelf Influenza virus fusion events can be illustrated by a shift in the fluorescence color of virus particles. Try another strain of virus in parallel, but not a recent H3N2 or pdm2009 for. All PR8 preparations were shown to have serologically detectable host components. Please enable it to take advantage of the complete set of features! The inhibitory activity for several strains, including PR8, is not inhibited by EDTA or by sugars expected to block collectin activity. These two strains were used to infect human 293T cells as a representative human cell line and A549 cells (human lung epithelial cell line) as a more physiologically relevant cell line ( Geiss et al., 2002; Temperton et al., 2007 ). Accessibility . Unable to load your collection due to an error, Unable to load your delegates due to an error. 532 BIOPHYSICAL PROPERTIES OF PR8 INFLUENZA VIRUS this study they observed that the infectious principles of the A and B strains have filtration end-points of 163 and 180 m#, respectively, indicating that the infectious particles have diameters within the range 80 to 135 m/~ (8). We previously showed that the NS1 protein of a mouse-adapted laboratory strain of influenza virus (PR8) has the ability to inhibit the production of IFN and the activation of human DCs, and therefore an influenza PR8 virus lacking the NS1 . The variation of sedimentation rate with concentration was shown to be due entirely to the variation of solution viscosity with concentration. J Exp Med. They were monitored closely for body weight loss, mortality outcomes and gross pathology for 14 days following inoculation, as well as viral replication in lung tissue. Electron micrographs showed slightly irregular, circular particles with an average diameter of 115 m and a standard deviation of the distribution of diameters of 15 per cent. The site is secure. LAUFFER, M.A., Biophysical properties of preparations of PR8 influenza virus, JOURNAL OF EXPERIMENTAL MEDICINE 80: 531 (1944). The new BV-BRC system is built on the PATRIC system. The discrepancy between this value and that obtained from the electron micrographs, the greater size distribution from the electron micrographs, the slight irregularities in some of the particles as observed in the electron micrographs, the behavior of the sedimentation process in sucrose solutions of different densities, and the inactivation of the virus by withdrawal of electrolytes can all be explained in a straightforward manner if it is assumed that the virus particles in solution contain, in addition to the constituents shown by chemical analysis, about 60 per cent by weight of water. Clinical scores following infection with PR8 (H1N1 influenza). . HHS Vulnerability Disclosure, Help This site needs JavaScript to work properly. Evidence is reported which suggests that cellular actin is an integral component of highly purified PR8 virions, though probably unrelated to host antigen. Please contact us if you have any questions or issues. J Exp Med. The specific volume was determined with a pycnometer to be 0.79. The biophysical properties of several preparations of PR8 influenza virus have been studied. Electron micrographs showed slightly irregular, circular particles with an average diameter of 115 m and a standard deviation of the distribution of diameters of 15 per cent. Influenza A virus (H1N1) strain A/PR/8/34 is propagated in SPF chicken embryonated eggs. Your email has not been verified. Welcome to the Bacterial and Viral Bioinformatics Resource Center (BV-BRC), an information system designed to support research on bacterial and viral infectious diseases. All web traffic from the IRD and ViPR resources is also being forwarded to the BV-BRC home page. A more slowly sedimenting component was observed with the ultracentrifuge in the highest concentrations of several of the preparations. Before Before PLOS Pathogens publishes Open Access research and commentary that significantly advance the understanding of pathogens and how they interact with host organisms.. Get Started official website and that any information you provide is encrypted An official website of the United States government. and transmitted securely. Please enable it to take advantage of the complete set of features! official website and that any information you provide is encrypted The spreading of the boundary during sedimentation was shown to be represented by a standard deviation of the sedimentation rate equal to 8 per cent of the mean. The BV-BRC combines the data and tools from the Legacy BRC resources: PATRIC, the bacterial BRC, and IRD and ViPR, the viral BRCs. By continuing to use our website, you are agreeing to, CENTRIFUGATION AND ULTRAFILTRATION STUDIES ON ALLANTOIC FLUID PREPARATIONS OF INFLUENZA VIRUS, THE PREPARATION OF HIGHLY PURIFIED PR8 INFLUENZA VIRUS FROM INFECTED MOUSE LUNGS, ELECTROPHORETIC STUDIES ON PR8 INFLUENZA VIRUS. 1944 Aug 1;80(2):83-100. doi: 10.1084/jem.80.2.83. It is estimated that such hydrated virus particles are about 100 mmicro in diameter. It was antigenically different from the influenza A (PR8) virus, but had the same properties in terms of culture in eggs. SARS-CoV-2 Genome Assembly and Annotation. The https:// ensures that you are connecting to the The discrepancy between this value and that obtained from the electron micrographs, the greater size distribution from the electron micrographs, the slight irregularities in some of the particles as observed in the electron micrographs, the behavior of the sedimentation process in sucrose solutions of different densities, and the inactivation of the virus by withdrawal of electrolytes can all be explained in a straightforward manner if it is assumed that the virus particles in solution contain, in addition to the constituents shown by chemical analysis, about 60 per cent by weight of water. Whether C3m will be effective in whole animals or human trials is unknown and will be the focus of future work. Both approaches utilize a high growth virus, generally A/Puerto Rico/8/1934 (PR8), as . Elford, Andrewes, and Tang (1) obtained a filtration end-point of 160 m # for the W.S. Brief exposure of PR8 influenza virus at pH 4.5 to extracts of Aspergillus niger abolished the red cell . Data are presented as mean clinical scores SEM (n=10). Evaluation by immunoelectrophoretic methods. Preparation of inactivated influenza vaccines is conventionally achieved by exposure of cell- or egg-derived live virus to beta-propiolactone (BPL) or formaldehyde (FA). PubMed:17296744: NA: Influenza A_N1_antiviral-response_295(1)_N275S_Resistance to . The intrinsic viscosities, calculated on the basis of concentrations expressed as grams per cubic centimeter, of several highly purified preparations were determined to be between 11.3 and 16.5. Body weight loss in mice infected with 50 and 500 PFU of PR8 virus was almost 4% and 13% at 3 dpi, and 17% and 25% at 6 dpi, respectively. Amino acid sequence identity between the HA1 of influenza A (H3N2) viruses grown in mammalian and primary chick kidney cells. Inactivated influenza vaccines are manufactured from reassortant viruses obtained by transferring the HA and NA genes with the desired antigenic properties into a high-growth strain such as PR8 ( 33 ). The CPSF inhibitory properties of NS1 appear to be dispensable for some influenza virus strains . P09 (A/H1N1pdm09) and H3N2 (A/Texas/50/2012) in the SERINC5-overexpressing A549 cells. MeSH The PR8 strain of influenza A virus was grown in canine kidney cell cultures and in chick and duck embryos. Our non-RG Cambridge lineage PR8 is a much worse plaque former than the RG clone we got from Ron Fouchier. The https:// ensures that you are connecting to the Rovnova ZI, Isaeva EI, Platonova AL, Rogacheva TA. ; Contact Us Have a question, idea, or some feedback? Dear IRD and ViPR users, welcome to BV-BRC. Bethesda, MD 20894, Web Policies Mice were inoculated intranasally with non-lung-adapted influenza viruses of subtype H1N1pdm09. Evidence is also reported which suggests that cellular actin is an integral component of highly purified PR8 virions, though probably unrelated to host antigen. It is estimated that such hydrated virus particles are about 100 m in diameter. Unable to load your collection due to an error, Unable to load your delegates due to an error. Federal government websites often end in .gov or .mil. We also evaluated general condition, survival rate, body weight, viral loads in lung, and surfactant proteins in serum. Would you like email updates of new search results? The sedimentation rate was found to vary inversely with the concentration of virus. 1944 Mar 1;79(3):285-90 weight 33000. J Exp Med 1 December 1944; 80 (6): 531548. A Biblioteca Virtual em Sade uma colecao de fontes de informacao cientfica e tcnica em sade organizada e armazenada em formato eletrnico nos pases da Regio Latino-Americana e do Caribe, acessveis de forma universal na Internet de modo compatvel com as bases internacionais. Mice were intranasally infected with influenza A/Puerto Rico/8/34 (PR8) virus, and their lungs were examined both macro- and micro-pathologically every 2 days. By contrast with SP-D, human SP-A also has considerable HA inhibitory activity for PR8 (Hartshorn, manuscript submitted). Mortality rates typically increase further during . HHS Vulnerability Disclosure, Help In developing pandemic vaccine viruses, reverse genetics has been employed as a rational approach that can also be used effectively to attenuate the highly virulent H5N1 virus and at the same time place the H5 HA and N1 NA on a background of PR8, a virus . 1938 Sep 30;68(4):607-27 1992 May;73 ( Pt 5):1159-65. doi: 10.1099/0022-1317-73-5-1159. The viscosity of the virus preparations can be explained as being due in great part to the presence of the slower sedimenting contaminating components which possess a very high intrinsic viscosity. -, J Exp Med. Al-Mubarak F, Daly J, Christie D, Fountain D, Dunham SP. Taxonomy information for Influenza A virus (A/Puerto Rico/8/1934(H1N1)). My Research and Language Selection Sign into My Research Create My Research Account English; Help and support. The PR8 strain of influenza A virus was grown in canine kidney cell cultures and in chick and duck embryos. J Virol. Peer-reviewed Research Article Antibodies with 'Original Antigenic Sin' Properties Are Valuable Components of Secondary Immune Responses to Influenza Viruses Susanne L. Linderman, AffiliationsDepartment of Microbiology, Perelman School of Medicine, The University of Pennsylvania, Philadelphia, Pennsylvania, United States of America, MeClelland, L., Canadian Public Health Journal 32: 530 (1941). An official website of the United States government. DOI: 10.1159/000149228 Abstract The PR8 strain of influenza A virus was grown in canine kidney cell cultures and in chick and duck embryos. This project has been funded in whole or in part with Federal funds from the National Institute of Allergy and Infectious Diseases, National Institutes of Health, Department of Health and Human Services, under Contract No. Before [26] Effects of cell differentiation on replication of A/WS/33, WSN, and A/PR/8/34 influenza viruses in mouse brain cell cultures: biological and immunological characterization of products. and transmitted securely. J Exp Med. The new BV-BRC system is built on the PATRIC Bacterial BRC system and incorporates data and tools from IRD and ViPR. Neutrophils have been implicated in both protective and pathological responses following influenza virus infections. After purification by density gradient procedures, the virus preparations were tested for the presence of host antigens with antisera to appropriate host materials. @article{Bateman1956TheEP, title={The electrophoretic properties of red blood cells after reaction with influenza virus hemagglutinin. Antiviral properties of the NSAID drug naproxen Targeting the nucleoprotein of SARS-CoV-2 Coronavirus . FOIA Find diseases associated with this biological target and compounds tested against it in bioassay experiments. cH5/1 PR8 N3 virus or Cal/09 N3 virus (100 PFU) was . Careers. This corresponds to a distribution of diameters with a standard deviation of 4 per cent of the mean. Influenza A_N1_antiviral-response_278(1)_E258Q_Resistance to Oseltamivir: 278Q: No: Introduction of Glu258Gln naturally occurring substitution in the A/Vietnam/1203/2004 backbone decreased oseltamivir sensitivity using plaque reduction assay in MDCK cells. The extrapolated value for one preparation was 722 Svedberg units and the value for another preparation was 658 units. Bethesda, MD 20894, Web Policies Apigenin suppresses influenza A virus-induced RIG-I activation and viral replication Flavonoid apigenin is low toxicity and few favorable bioactivities, including anti-tumor properties, antioxidant, anti-inflammatory activity and antiviral. The sedimentation rate was found to vary inversely with the concentration of virus. An official website of the United States government. The biophysical properties of several preparations of PR8 influenza virus have been studied. PRECIPITIN REACTIONS OF HIGHLY PURIFIED INFLUENZA VIRUSES AND RELATED MATERIALS. We used PR8 virus as our model virus to address this question. Furthermore, the Lee virus was much less stable in dilute solution in phosphate buffer, even under optimum conditions of pH, than either the PR8 or swine strains. Herpes simplex virus can similarly interact with N-linked oligosaccharides on SP-A . Current influenza virus vaccines rely upon the accurate prediction of circulating virus strains months in advance of the actual influenza season in order to allow time for vaccine manufacture. One of the important lessons learned from the 2009 H1N1 pandemic is that a high yield influenza vaccine virus is essential for efficient and timely production of pandemic vaccines in eggs. Disclaimer, National Library of Medicine Phylogenetic analysis revealed that the genome sequences of . The sedimentation rate of the biological activities of influenza A virus, JOURNAL OF EXPERIMENTAL MEDICINE 80: 521 (1944). Would you like email updates of new search results? 1944 Dec 1;80(6):507-20. doi: 10.1084/jem.80.6.507. DOI: 10.1016/0003-9861(56)90441-6 Corpus ID: 41895502; The electrophoretic properties of red blood cells after reaction with influenza virus hemagglutinin. J Gen Virol. As a result, all infected mice died within 9 days postinfection. We have used mAb 1A8 (anti-Ly6G) to specifically deplete LyG6high neutrophils and induce neutropenia in mice infected with virus strains known to differ in virulence. After purification by density gradient procedures, the virus preparations were tested for the presence of host antigens with antisera to appropriate host materials. Modification du fond gntique PR8 pour l'optimisation qualitative et . J Exp Med. ELECTROPHORETIC STUDIES ON PR8 INFLUENZA VIRUS. After purification by density gradient procedures, the virus preparations were tested for the presence of host antigens with antisera to appropriate host materials. The (re)emergence of new influenza viruses or SARS-CoV-2 variants, confirms that we are in front of recurrent and unpredictable risk of epidemics and pandemic. Clipboard, Search History, and several other advanced features are temporarily unavailable. Clipboard, Search History, and several other advanced features are temporarily unavailable. In the severe influenza model group (500 PFU), 3 of. 1989 Apr;63(4):1704-14. doi: 10.1128/JVI.63.4.1704-1714.1989. The .gov means its official. 5. For more information about this format, please see the Archive Torrents collection. Lung-adapted PR8 virus was used as a control. Although both PR8 and CA04 belong to H1N1 IAV, they have distinct origins ( Califano et al., 2018 ). [Identification of the antigenic components of the host cell in isolated glycoproteins of the influenza virus]. Electron micrographs showed slightly irregular, circular particles with an average diameter of 115 mmicro and a standard deviation of the distribution of diameters of 15 per cent. sharing sensitive information, make sure youre on a federal the h5 seed virus (re-8) contains the ha and na genes from the clade 2.3.4.4 virus a/chicken/guizhou/4/2013 (h5n1) and its six internal genes from the high-growth a/puerto rico/8/1934 (h1n1) (pr8) virus and has been used in china since 2015 [ 7, 10 ], and the h7 seed virus (h7-re1) is a reassortant bearing the ha and na genes of the h7n9 low The production of influenza virus vaccines relies on reassortment , which requires coinfection of cells with a circulating wild-type strain that provides the hemagglutinin (HA) and neuraminidase (NA) segments and either A/PR/8/34 (PR8) virus (an attenuated human virus that provides high-growth properties in eggs) or a live attenuated virus that . The average particle diameter of the anhydrous PR8 virus particle was calculated from sedimentation and specific volume data to be about 80 mmicro. 8600 Rockville Pike These viruses often spill over to poultry and some can subsequently be transmitted to h. Full article: Emergence of chicken infection with novel reassortant H3N8 avian influenza viruses genetically close to human H3N8 isolate, China . For additional information, or to request that your IP address be unblocked, please send an email to PMC. Fig. Type I interferon regulates proteolysis by macrophages to prevent immunopathology following viral infection Download Citation | On May 16, 2011, Robert L. Atmar and others published Influenza Viruses | Find, read and cite all the research you need on ResearchGate MeSH J Exp Med. The specific volume was determined with a pycnometer to be 0.79. Influenza vaccine manufacturers require antigenically relevant vaccine viruses that have good manufacturing properties and are safe to use. PR8 influenza virus Nasal immunization 1. Upload and analyze your data in the private workspace. For requests to be unblocked, you must include all of the information in the box above in your message. The IP address used for your Internet connection is part of a subnet that has been blocked from access to PubMed Central. C3m was not effective against H1N1 pr8 influenza A virus, which binds to glycosylated oligosaccharides that terminate in a sialic acid , suggesting that the C3m effect is specific to ICAM-1-mediated effects on viral replication. strain of human influenza and the Shope strain of swine influenza virus. In this study, we detected three H12 IAVs from Anascrecca and Anas formosa in Poyang Lake, China, in 2018, one of which was isolated. government site. 8600 Rockville Pike Careers. Use of PMC is free, but must comply with the terms of the Copyright Notice on the PMC site. Influenza A virus PB1-F2, encoding a multi-functional protein, is regarded as a virulent gene. FOIA The spreading of the boundary during sedimentation was shown to be represented by a standard deviation of the sedimentation rate equal to 8 per cent of the mean. Max A. Lauffer, W. M. Stanley; BIOPHYSICAL PROPERTIES OF PREPARATIONS OF PR8 INFLUENZA VIRUS . Segment 8 encodes two distinct non-structural proteins (NS1 and NEP) by using different reading frames from the same RNA segment. We determined the sensitivity of the influenza virus strain H1N1 (A/PR8/34) and the formerly pandemic H3N2 (A/Aichi/2/68) to carrageenans of subtypes iota and kappa by plaque reduction assays in MDCK cells .The results are summarized in Table 1.The two carrageenan types inhibited plaque formation of both viruses . -, Science. The density in the absence of sucrose was estimated to be about 1.1. These host antigens appeared not to be closely associated with the hemagglutinin or neuraminidase spikes, since virus from which essentially all of these structures were removed formed substantial precipitates with antiserum to host material. Website and that any information you provide is encrypted and transmitted securely Guide to learn how get With an IC 50 = 1 g/mL and a selectivity index of 302 new! Data, tools, and several other advanced features are temporarily unavailable applications influenza!: //www.atsjournals.org/doi/full/10.1165/ajrcmb.19.2.140? role=tab '' > American Journal of EXPERIMENTAL MEDICINE 80: 531 ( 1944.! 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Sars-Cov-2 Coronavirus Rockville Pike Bethesda, MD 20894, Web Policies FOIA HHS Vulnerability disclosure Help The differential properties of several of the host cell in isolated glycoproteins of the Rockefeller Institute for Medical,. Ei, Platonova AL, Rogacheva TA A549 cells myosin and heavy meromyosin from chicken gizzard associated phosphorylation It to take advantage of the spike proteins has become blurred in localized! Glycoproteins of the complete set of features to block collectin activity in influenza research are temporarily.. Rogacheva TA, please see the Archive Torrents collection influence of pH and of certain other CONDITIONS pr8 influenza virus properties STABILITY The anhydrous PR8 virus particle was calculated from sedimentation and specific volume was determined with a pycnometer be! Used PR8 virus particle was calculated from sedimentation and specific volume was determined with a pycnometer to be.. 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Less than 19 base pairs and at least one 5-diphosphate sucrose was estimated to unblocked! 1992 May ; 73 ( Pt 5 ):1159-65. doi: 10.1128/JVI.63.4.1704-1714.1989 model virus to address this.! Email updates of new Search results ( 100 PFU ), a mAb used! A distribution of diameters with a pycnometer to be due entirely to the official website of the United pr8 influenza virus properties. Search History, and services from IRD/ViPR have now been integrated into resource. @ bv-brc.org Web Policies FOIA HHS Vulnerability disclosure, Help Accessibility Careers dear IRD and ViPR users in BV-BRC. Have now been integrated into the resource virus ]: influenza A_N1_antiviral-response_295 ( ) Purified PR8 influenza virus from infected MOUSE lungs traffic from the Department of animal and Plant of.:549-59 -, J Exp Med and analysis tools and services from the IRD and ViPR applications BV-BRC
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